Research Profile: Imaging fixed and embedded tissues with MALDI MS
نویسندگان
چکیده
منابع مشابه
High-resolution MALDI-FT-ICR MS imaging for the analysis of metabolites from formalin-fixed, paraffin-embedded clinical tissue samples.
We present the first analytical approach to demonstrate the in situ imaging of metabolites from formalin-fixed, paraffin-embedded (FFPE) human tissue samples. Using high-resolution matrix-assisted laser desorption/ionization Fourier-transform ion cyclotron resonance mass spectrometry imaging (MALDI-FT-ICR MSI), we conducted a proof-of-principle experiment comparing metabolite measurements from ...
متن کاملMALDI imaging of formalin-fixed paraffin-embedded tissues: application to model animals of Parkinson disease for biomarker hunting.
A common technique for the long-term storage of tissues in hospitals and clinical laboratories is preservation in formalin-fixed paraffin-embedded (FFPE) blocks. Such tissues stored for more than five years have not been useful for proteomic studies focused on biomarker discovery. Recently, MS-based proteomic analyses of FFPE showed positive results on blocks stored for less than 2 days. Howeve...
متن کاملMALDI Imaging MS: Molecular Visualization of Tissues in Drug Discovery and Development
Our current approach to risk assessment in the development of safe and efficacious drugs is primarily based on the parent drug plasma exposure (area under the concentration/time curve; AUC) in both animal models and in humans [1]. The successful implementation of this strategy is driven by a robust analytical methodology: LC-MS/MS. One of the key advantages of this “exposure-centric” approach i...
متن کاملDirect analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections.
Formalin fixation, generally followed by paraffin embedding, is the standard and well-established processing method employed by pathologist. This treatment conserves and stabilizes biopsy samples for years. Analysis of FFPE tissues from biopsy libraries has been, so far, a challenge for proteomics biomarker studies. Herein, we present two methods for the direct analysis of formalin-fixed, paraf...
متن کاملImaging of N-Linked Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using MALDI Mass Spectrometry
Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling of N-glycans on tissue sections can reveal tissue-specific and/or disease-associated N-glycans, which not only could serve as molecular signatures for diagnosis but also shed light on the functional roles of these biomolecules. Mass spectrometry imaging (MSI) is a powerful tool that has been used to cor...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2007
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr070736w